Mutation analysis of the EGFR gene

Instructions for the patient

Patients with non-small cell lung cancer (N.S.C.L.C.) show a significant increase in their survival rates after treatment with monoclonal drugs (gefitinib, erlotinib) which inhibit the activity of the EGFR gene and block its expression. However, only patients presenting with mutations of the EGFR gene obtain the greatest benefit from the treatment; patients with non-mutated (wild-type) EGFR obtain only reduced benefit or no benefit at all. Accurate determination of EGFR status (i.e. mutated or non-mutated) is therefore fundamental in deciding which patients to treat.

Material to be provided

  • Inclusion in paraffin or unstained histological slides relating to the histological examination of the tumour. This material should be requested from the healthcare unit in which the diagnostic histological examination was carried out. Please ensure that the unit receives the attached form and returns it together with the material provided for analysis.
  • Copy of the diagnostic histological report.

If either of the above items is missing, the evaluation may be incomplete and in some cases the test may not be carried out.

Please note: for diagnostic histological examinations that have been carried out at the Campus Bio-Medico University Hospital, the request itself is sufficient and neither slides nor a copy of the report need be sent (Red order form).

Request for specialist SSN Services (The Italian National Health Services) (Red order form)

The form used to request the analysis must be filled out by an SSN physician with a copy filed in the relevant Outpatient Clinic’s Analysis Request Register. The request should include the words:

a. “Estrazione di DNA o di RNA”
b. “Conservazione di campioni di DNA o RNA”
c. “Ricerca mutazioni gene EGFR: analisi di segmenti di DNA mediante sequenziamento (05 sequenze)”

In order for us to be able to keep in touch regarding the service requested, the referring physician contact telephone number should be included.

Delivery details

a)  The red SSN order form used to arrange a EGFR gene mutation analysis should be delivered to the Outpatients Clinic reception desk (First Underground Level), from Monday to Friday, between 8 am and 10 am.

b) he material submitted for analysis (whether inclusion or slides) should be delivered to the Pathological Anatomy Laboratory (First Floor, East Wing) together with:

  • a copy of the histological report
  • the attached form duly completed
  • a photocopy of the red SSN order form  (issued by the staff of the Outpatients Clinic reception desk of the Campus Bio-Medico University Hospital when they are logging in the request for the examination)

The Pathological Anatomy Laboratory is opened for deliveries from Monday to Friday, from 7.30 am to 12.30 pm and from 2 pm to 4.30 pm. For further information regarding the examination please telephone 06 22541 1151 during the times shown above or send an e-mail.

Collecting the report

Reports will be available for collection 20 working days after the request is submitted. Either the patient or a delegated person can collect the report at the Medical Documentation Archive.